HIV1 is able to infect resting TCD4+ cells efficiently in a short time of viral exposure
Abstract number: P2165
Maricato J.T., Alkmim W., Fincatti P., Nascimento-Brito S., Machado Jr. J., Janini L.
HIV is the causative agent of the acquired immunodeficiency syndrome (AIDS). According to UNAIDS there are roughly 33 million people living with HIV. Several studies have addressed many aspects of HIV infections but elements pertaining to the relationship between the virus and target cells are still waiting for a better characterization. Most of the studies on early steps of viral infection showed that cellular activation is essential for HIV infection in TCD4+ lymphocytes and monocytes. The objective of the this work was to determinate if HIV-1 is able to infected resting TCD4+ lymphocytes purified from human peripheral blood. The virus was able to infect resting TCD4+ lymphocytes on MOIs ranging from 0,05 to 1,0. Our preliminary results showed that resting TCD4+ lymphocytes can be infected by HIV-1 within 30 minutes after the addition of virus to cell cultures. After infections, HIV-1 reverse transcription was allowed to proceed for 36h under IL-2 and PHA activation. Conventional PCR and RFLP of the protease gene showed that viral DNA can be detected for all MOIs and in all time periods from 30 minutes up to 180 minutes post-infection. Proviral load will be measured by qPCR for each MOI and period infection. Our results indicated that resting TCD4+ cells can be efficiently infected by HIV when different MOIs are used and even when cells were exposed to virus for only 30 minutes. Infection of resting cells does not represent a barrier for HIV since virus could be recovered 36 hours post infection. Our data may have some implications on how HIV can manage to establish infection on new hosts.
|Session name:||Abstracts of 21st ECCMID / 27th ICC|
|Location:||Milan, Italy, 7 - 10 May 2011|
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