Antimicrobial resistance of H.pylori in Smolensk, Russia
Abstract number: P1042
Dekhnich N., Kostykova E., Punin A., Alimov A., Ivanchik N., Kozlov R.
Objectives: To evaluate the susceptibility to antimicrobials of H. pylori isolated from patients with H. pylori-associated gastrointestinal disorders in Smolensk (Russia).
Methods: Overall 210 adult patients with H. pylori-associated gastrointestinal disorders were included in the study in 20092010. From each patient 2 antral and 2 corpus gastric biopsy samples were taken for culture and placed in a phosphate buffer (Sigma, USA) or Portagerm pylori (BioMerieux, France) for transportation to the laboratory. Pylori agar (BioMerieux, France) plates were incubated for 35 days at 37°C under microaerophilic conditions (9% CO2, 80% N2, 11% O2). Antimicrobial susceptibility testing was performed on Mueller-Hinton agar (BBL, USA) supplemented with 5% sheep blood by the dilution method to amoxicillin, erythromycin, clarithromycin, levofloxacin, ciprofloxacin, tetracycline and metronidazole.
Results:H. pylori were isolated in 64% (n = 135) patients. Among them 18% were resistant to one antimicrobial agent, 3% were resistant to more than one antimicrobial agent. Seven (5.3%) isolates were resistant to clarithromycin (MIC 1 mg/L), 10 (7.6%) to erythromycin (MIC >4 mg/L). The resistance rate of H. pylori to metronidazole was 3.8% (n = 5) (MIC >4 mg/L). No strains resistant to amoxicillin and tetracycline were found. Resistance to ciprofloxacin was detected in 12.8% (n = 17) (MIC >1 mg/L) of strains, to levofloxacin in 8.3% (n = 11) (MIC >1 mg/L).
Conclusion: Our study highlights the importance of antibiotic resistance surveillance to guide test and treat policies. In Smolensk we can use current recommendations of the European H. pylori study group (proton pump inhibitor + clarithromycin + amoxicillin or metronidazole) as the initial treatment for H. pylori infection. Quadruple therapy (proton pump inhibitor + bismuth + metronidazole + tetracycline) could be an alternative initial therapy.
|Session name:||Abstracts of 21st ECCMID / 27th ICC|
|Location:||Milan, Italy, 7 - 10 May 2011|
|Back to top|