Serotype distribution and antimicrobial susceptibility of Streptococcus pneumoniae isolated from children with invasive pneumococcal infections in Turkey
Abstract number: P962
Nar Otgun S., Durmaz R., Gozalan A., Guldemir D., Acar B., Ertek M.
Objectives: The aim of this study was to determine the serotype distribution and antimicrobial susceptibility of Streptococcus pneumoniae strains isolated from invasive infections among children younger than five years of age in Turkey after routine vaccination with seven-valent conjugated pneumococcal vaccine (PCV-7).
Methods: In the scope of this multi-center study, a total of 287 clinical samples or strains isolated from the invasive infections were examined in our laboratory from May 2009 to December 2010. Isolates were serotyped by quellung reaction with the antisera panel (Statens Serum Institute, Copenhagen, Denmark) and sequential multiplex PCR. Susceptibilities to penicillin, cephotaxim, meropenem and vancomycin of the isolates were determined by E-test (AB Biodisk, Solna, Sweden).
Results: A total of 60 pneumococcal strains were recovered from the all study group. The most frequent serotypes were 19F (20%), 3 (8,3%), 14 (6,7%) and 1 (6,7%). Thirty two of these strains were isolated among children 5 years old. And also pneumococcal DNA were detected by sequential multiplex PCR directly from the cerebrospinal fluid of three cases in this age group. A total of 20 different serotypes/serogroups were found among children 5 years old, the most frequent being 19F (20% of the strains), Pool I (8,6%), 6A (8,6%), 18C (5,7%), 9L/N (5,7%), 5 (5,7%%), 3 (5,7%), 1 (5,7%). Vaccine serotype coverages were 34,4%, 48,7%, 65,9% for PCV-7, 10- and 13-valent pneumococcal conjugate vaccines, respectively. Twenty two strains (68,75%) were susceptible to penicillin, 7 (21,87%) were intermediate and 3 (9,37%) were resistant. All strains were susceptible to cephotaxim, meropenem and vancomycin except one isolate. This one was intermediate resistant to cephotaxim and meropenem, resistant to penicillin although it was susceptible to vancomycin.
Conclusions: We have gained some insight on the serotypes and susceptibility of invasive S. pneumoniae strains in Turkey following to PCV-7 vaccination. We consider that this multi-center study is a good background for monitoring the epidemiology of invasive pneumococcal diseases in Turkey.
|Session name:||Abstracts of 21st ECCMID / 27th ICC|
|Location:||Milan, Italy, 7 - 10 May 2011|
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